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Year : 2014  |  Volume : 16  |  Issue : 2  |  Page : 61-66

Detection of Yersinia enterocolitica in diarrhoiec stools and environmental samples

1 Department of Microbiology, Neurofoundation Hospital, Salem, India
2 Department of Microbiology, Velammal Medical College Hospital and Research Institution, Madurai, Tamil Nadu, India
3 Department of Microbiology, Jagadguru Sri Shivarathreeswara Medical College, Mysore, Karnataka, India

Correspondence Address:
V Suneetha
Department of Microbiology, Neurofoundation Hospital, Salem
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0972-1282.144710

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Aim: To detect distribution of Yersinia enterocolitica in gastroenteritis cases, in environmental samples and food samples consumed by humans. Materials and Methods: Stool samples, environmental samples comprising water from water pools on the bank of river 'Kabini,' fruit juices from commercial fresh juice centers, pork intestinal contents, drinking water from public eateries, ('paani puri' outlets), public tap water samples and commercial prepacked juices. All samples were cultured by four different methods; direct plating on MacConkey agar, plating following cold enrichment, single alkali and double alkali treatment with KOH & PBS. Results: A total of 181 stool samples and 15 food and environmental samples were processed. Y. enterocolitica was isolated from one stool sample (0.55%) by cold enrichment method and from one water sample collected from river bank (6.6%). Conclusion: Y. enterocolitica is found in humans and in the environment in this region. Adopting a rapid isolation method may infuse interest among clinical microbiologists to undertake an epidemiological study on Y. enterocolitica and Yersiniosis. Unhygienic defaecation habits of the people and movement of swine herds along the river bank may be the source for river water contamination.

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