Background: Arboviruses are arthropod-borne viruses transmitted during the blood meal of the arthropod. These arboviruses are considered to be emerging and reemerging infections. Aims and objectives: This survey is being conducted to determine the testing capacity of various hospitals and laboratories for arboviral infections, their testing methods, and positivity rate. Materials and methods: This questionnaire-based study was conducted after obtaining ethical clearance. The questionnaire was circulated via email, WhatsApp, or other electronic modes to clinical microbiologists in India. Data were collected on testing for dengue, chikungunya, Japanese encephalitis (JE), Kyasanur forest disease (KFD), Zika, and West Nile virus from June 2022 to May 2023 (1 year). The collected data were tabulated and analyzed using frequency and percentages. Results: A total of 39 testing facilities responded to the questionnaire. Among these facilities, 20 (51.28%) were from Kerala, and 26 (66.6%) were teaching hospitals, of which 15 (38.46%) were private. Two (5.1%) were stand-alone private laboratories. Rapid diagnostic tests (RDTs), immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA), and reverse transcription polymerase chain reaction (RT-PCR) for ribonucleic acid (RNA) detection were the common tests performed. A total of 1,07,535 samples were tested for the six major arboviral infections, with dengue accounting for 93.59% of the tests conducted. All these diseases are reportable under the Integrated Disease Surveillance Programme (IDSP). Facility for viral culture and isolation, considered the gold standard for diagnosing viral diseases, was not available at any of the centers. Conclusion: This survey is the first of its kind to examine arboviral disease testing capacity across 39 diagnostic facilities in India. Dengue diagnosis was the most common among the arboviral diseases tested. However, paradoxically, tests for potentially fatal viral infections, like Japanese encephalitis virus (JEV), and IgM ELISA for KFD, are only available at referral centers. This study highlights the need for improved capacity building for arboviral disease detection across the country. Early diagnosis is crucial for controlling the spread of these viruses in communities.