Evaluation of conventional and CHROMagar method for the detection of Group B Streptococcus in antenatal cases
Vijaya Doddaiah, Vijaya Shivanna, S Vijayalakshmi, ST Santhya, R Shakthi
Keywords :
CHROMagarTM Strep B agar, Group B Streptococcus, latex agglutination
Citation Information :
Doddaiah V, Shivanna V, Vijayalakshmi S, Santhya S, Shakthi R. Evaluation of conventional and CHROMagar method for the detection of Group B Streptococcus in antenatal cases. 2016; 18 (2):127-130.
Background: Group B Streptococcus (GBS) has reemerged as a major pathogen during the past few decades. Newborns with early-onset GBS disease acquire infection from the maternal genital tract. The aim of the present study was to find the prevalence of GBS among antenatal cases and to evaluate the conventional and CHROMagarTM Strep B agar method in the detection of GBS colonization among pregnant women.
Materials and Methods: A total of 160 vaginal swabs were collected from pregnant women of 35-37 weeks of gestation and inoculated onto 5% sheep blood agar and CHROMagarTM Strep B agar. GBS grown on 5% sheep blood agar and CHROMagarTM Strep B agar were confirmed by biochemical and latex agglutination tests.
Results: GBS was detected in 14.38% of pregnant women. CHROMagarTM Strep B agarshowed 100% sensitivity and specificity in comparison with the conventional method.
Conclusion: In the present study, GBS was prevalent in 14.38% of the antenatal cases. CHROMagarTM Strep B agar with 100% sensitivity and specificity can be used to screen all pregnant women for GBS colonization as it does not require expertise in identification.
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