Journal of The Academy of Clinical Microbiologists

Register      Login

VOLUME 16 , ISSUE 2 ( July-December, 2014 ) > List of Articles

Original Article

Detection of Yersinia enterocolitica in diarrhoiec stools and environmental samples

V Suneetha, G. S. Vijay Kumar, M. Raghavendra Rao

Keywords : Cold enrichment, chitterling, Yersiniosis, Zoonotic

Citation Information : Suneetha V, Kumar GS, Rao MR. Detection of Yersinia enterocolitica in diarrhoiec stools and environmental samples. 2014; 16 (2):61-66.

DOI: 10.4103/0972-1282.144710

License: CC BY-NC-SA 4.0

Published Online: 15-07-2024

Copyright Statement:  Copyright © 2014; Wolters Kluwer India Pvt. Ltd.


Abstract

Aim: To detect distribution of Yersinia enterocolitica in gastroenteritis cases, in environmental samples and food samples consumed by humans. Materials and Methods: Stool samples, environmental samples comprising water from water pools on the bank of river ‘Kabini,’ fruit juices from commercial fresh juice centers, pork intestinal contents, drinking water from public eateries, (‘paani puri’ outlets), public tap water samples and commercial prepacked juices. All samples were cultured by four different methods; direct plating on MacConkey agar, plating following cold enrichment, single alkali and double alkali treatment with KOH & PBS. Results: A total of 181 stool samples and 15 food and environmental samples were processed. Y. enterocolitica was isolated from one stool sample (0.55%) by cold enrichment method and from one water sample collected from river bank (6.6%). Conclusion: Y. enterocolitica is found in humans and in the environment in this region. Adopting a rapid isolation method may infuse interest among clinical microbiologists to undertake an epidemiological study on Y. enterocolitica and Yersiniosis. Unhygienic defaecation habits of the people and movement of swine herds along the river bank may be the source for river water contamination.


PDF Share
  1. Laukkanen R, Hakkinen M, Lundén J, Fredriksson-Ahomaa M, Johansson T, Korkeala H. Evaluation of isolation methods for pathogenic Yersinia enterocolitica from pig intestinal content. J Appl Microbiol 2010;108:956-64.
  2. Bottone EJ. Yersinia enterocolitica: The Charisma continues. Clin Microbiol Rev 1997;10:257-76.
  3. Hayashidani H, Ishiyama Y, Okatani TA, Yoshida S, Ishikawa M, Kato Y, et al. Molecular genetic typing of Yersinia enterocolitica serovar O:8 isolated in Japan. Adv Exp Med Biol 2003;529:363-5.
  4. Fredriksson-Ahomaa M, Stolle A, Stephan R. Prevalence of pathogenic Yersinia enterocolitica in pigs slaughtered at a Swiss abattoir. Int J Food Microbiol 2007;119:207-12.
  5. Annamalai T, Venkitanarayanan K. Expression of major cold shock proteins and genes by Yersinia enterocolitica in synthetic medium and foods. J Food Prot 2005;68:2454-8.
  6. Taxonomy, enterobacteriaceae. In: Konemann WE, Stephen, Allan D, Janda WM, Fehreckenberger CP, Winn WC, editors. Koneman's Color Atlas and Text book of Diagnostic Microbiology. 6th ed. Philadelphia: Lipincott's Publications. p. 273-4.
  7. Joyce D, Coghlan, Yersinia, Pateurella, Franciella. In: Collee JG, Fraser AG, Marmion B P, Simmons A, editors. Mackie and Mc Cartney Practical Medical Microbiology. 14th ed. London: Churchill Livingstone; 1996. p. 479-87.
  8. Sinha I, Choudhary I, Virdi J. Isolation of Yersinia enterocolitica and Yersinia intermedia from waste waters and their biochemical and serological characteristics. Curr Sci 2000;79:510-73.
  9. Singh I, Virdi JS. Isolation, bio characterization and in vitro test pathogenicity of Yersinia enterocolitica isolated from pork. Curr Sci 1999;77:1019-21.
  10. The Enterobacteria. In: Barrow GI, Feltham RK, editors. Cowan and Steel's Manual for Identification of Medical Bacteria. 3 rd ed. Canbridge; 1993. p. 78-9.
  11. Centers for Disease Control and Prevention. Yersinia enterocolitica. CDC. Available from: http://www.cdc.gov/ncidod/dbmd/diseaseinfo/yersinia_g.htm [Last accessed on 2014 July 26].
  12. Schiemann DA. Synthesis of a selective agar medium for Yersinia enterocolitica. Can J Microbiol 1979;25:1298-304.
  13. Singh G, Arora NK, Bhan MK, Ghai OP, Dhar S, Shriniwas DC. Role of Yersinia enterocolitica in childhood diarrhea. Indian J Paediatr 1983;50:39-42.
  14. Varghese A, Ramachandran VG, Agarval DS. Isolation and characterization of Yersinia enterocolitica from patients of gastroenteritis. Indian J Med Res 1984;79:35-40.
  15. Onyemelukwe NF. Yersinia enterocolitica as an aetiological agent of childhood diarrhea in Enugu, Nigeria. Cent Afr J Med 1993;39:192-5.
  16. Lal M, Kaur H, Gupta LK. Y. Enterocolitica gastroenteritis-a prospective study. Indian J Med Microbiol 2003;21:186-8.
  17. Ram S, Khurana S, Singh R, Sharma S, Vadehra DV. Yersinia enterocolitica diarrhea in North India. Indian J Med Res 1987;86:9-13.
  18. Singh, Bhatnagar S, Virdi JS. Isolation and characterization of Yersinia enterocolitica from diarrhoeic human subjects and other sources. Curr Sci 2003;84:1353-5.
  19. Greenwood JR, Flanigan SM, Pickett MJ, Martin JW. Clinical isolation of Yersinia enterocolitica: Cold temperature enrichment. J Clin Microbiol 1975;2:559-60.
  20. Centers for Disease Control and Prevention (CDC). Yersinia enterocolitica gastroenteritis among infants exposed to chitterlings-Chicago, Illinois, 2002. MMWR Morb Mortal Wkly Rep 2003;52:956-8.
  21. el-Sherbini M, al-Agili S, el-Jali H, Aboshkiwa M, Koha M. Isolation of Yersinia enterocolitica from cases of acute appendicitis and ice-cream. East Mediterr Health J 1999;5:130-5.
  22. Abraham M, Pai M, Kang G, Asokan GV, Magesh SR, Bhattacharji S, et al. An outbreak of food poisoning in Tamil Nadu associated with Yersinia enterocolitica. Indian J Med Res 1997;106:465-8.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.