Aim: This study was conducted to find out the bacterial aetiological agents of burn wound infections with antimicrobial testing of the bacterial isolates. Materials and Methods: This is a descriptive study of 203 patients with burn wound infections at Government Medical College, Thiruvananthapuram during the period January 2010 to April 2011. The specimens were cultured using aerobic microbiological techniques. Antimicrobial susceptibility testing to different agents was carried out using the disc diffusion method. Results: Cultures from burn wound revealed Pseudomonas aeruginosa as the most common organism followed by Staphylococcus aureus, Escherichia coli, Acinetobacter baumannii and Klebsiella pneumoniae. There was a high rate of multidrug-resistant organisms. However, all the staphylococci were susceptible to Vancomycin and the gram negatives were susceptible to Carbapenems. Conclusion: Collection of appropriate specimens for culture before starting of antibiotics helps in better management, along with isolation precautions. However close follow-up and repeat specimens are necessary for appropriate change in antibiotics. For empiric treatment Vancomycin and Imipenem appear to be a good combination in this hospital.