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VOLUME 15 , ISSUE 2 ( July-December, 2013 ) > List of Articles

Original Article

Comparison of various methods for detection of AmpC β-lactamase enzyme

Vrushali Thakar, Meera Modak

Keywords : AmpC-b, disc test, lactamase, three-dimensional enzyme extract test

Citation Information : Thakar V, Modak M. Comparison of various methods for detection of AmpC β-lactamase enzyme. 2013; 15 (2):45-48.

DOI: 10.4103/0972-1282.124585

License: CC BY-NC-SA 4.0

Published Online: 20-07-2024

Copyright Statement:  Copyright © 2013; Wolters Kluwer India Pvt. Ltd.


Abstract

Fifty multidrug-resistant bacterial isolates were tested for extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase enzyme production. Cefoxitin (30 μg) resistance was used as the screening test for AmpC enzyme detection. Phenotypic confirmation was done by conventional three-dimensional enzyme extract test (TDET), AmpC disc test and modified three-dimensional test (MDT). A total of 45 (90%) strains were AmpC positive by the screening test. Out of these 45 strains, 40 strains were positive by AmpC disc test, 39 strains by TDET and 38 strains by MDT. Cefoxitin resistance can be used as the screening test. AmpC disc test is simple to perform and gives rapid result. So, it can be used as a phenotypic method for detection of AmpC enzyme production in resource-limited settings where genotypic detection methods are not available.

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  1. AmpC disk test for detection of plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae lacking chromosomal AmpC beta-lactamases. J Clin Microbiol 2005;43:3110-3.
  2. AmpC beta-lactamase producing bacterial isolates from Kolkata Hospital. Indian J Med Res 2005;122:224-33.
  3. Phenotypic detection of extended spectrum and AmpC beta-lactamases by a new spot-inoculation method and modified three-dimensional extract test: Comparison with the conventional three-dimensional extract test. J Antimicrob Chemother 2004;54:684-7.
  4. Occurrence of ESBL & Amp-C beta-lactamases & susceptibility to newer antimicrobial agents in complicated UTI. Indian J Med Res 2008;127:85-8.
  5. Prevalence of extended spectrum beta lactamase and AmpC beta lactamase producers among Escherichia coli isolates in a tertiary care hospital in Jaipur. Indian J Pathol Microbiol 2008;51:367-9.
  6. Molecular cloning. A Laboratory Manual. Vol 3. 3 rd ed. Cold Spring Harbor Laboratory Press, New YORK; 2001. p. A1.7.
  7. Evaluation of methods for AmpC beta-lactamase in gram negative clinical isolates from tertiary care hospitals. Indian J Med Microbiol 2005;23:120-4.
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