|Year : 2015 | Volume
| Issue : 2 | Page : 89-93
Amoebic vs pyogenic liver abscesses: A comparative study in a tertiary care hospital
Ardra R Menon, Pushpa K Kizhakkekarammal, Girija K Rao
Department of Microbiology, Government Medical College, Thrissur, Kerala, India
|Date of Web Publication||15-Dec-2015|
Ardra R Menon
Department of Microbiology, Government Medical College, Thrissur, Kerala
Source of Support: None, Conflict of Interest: None
Background and Objectives: Liver abscess is a potentially life-threatening condition with a majority of cases being amoebic in tropical countries. In India, poor sanitary conditions and low socioeconomic status contribute to the endemicity of amoebiasis. As treatment modalities are different for amoebic and pyogenic liver abscesses (ALA and PLA, respectively), it is necessary to differentiate them. Facilities for amoebic culture are not available and microscopy is nondiscriminatory, hence serological tests are greatly relied upon for the identification. No studies have been done so far in our region on patients admitted with liver abscess. The aims of this study were to find the aetiology of liver abscess in admitted patients and to know the proportion of amoebic liver abscess using serological techniques. Materials and Methods: The study was done for 6 months and the sample size was 50. The ultrasonography (USG)-guided aspirates were subjected to microscopy and aerobic and anaerobic cultures on appropriate media. Serum samples of all patients were examined for Entamoeba histolytica immunoglobulin G (IgG) antibodies by enzyme-linked immunosorbent assay (ELISA) along with 35 control samples. Results: Of the 50 patients, 34 were diagnosed as amoebic by (ELISA) (68%), 11 as pyogenic (22%) and five as indeterminate (10%). Alcohol was found to have a higher association with ALA than with PLA. Of the 34 amoebic liver abscesses, only four (8.8%) showed motile trophozoites. The mortality rate was only 2.2%. Conclusion: Rapid diagnosis with serology and prompt treatment can result in reduced hospital stay in cases of ALA.
Keywords: Amoebic, enzyme-linked immunosorbent assay (ELISA), Entamoeba histolytica, liver abscess, pyogenic
|How to cite this article:|
Menon AR, Kizhakkekarammal PK, Rao GK. Amoebic vs pyogenic liver abscesses: A comparative study in a tertiary care hospital. J Acad Clin Microbiol 2015;17:89-93
|How to cite this URL:|
Menon AR, Kizhakkekarammal PK, Rao GK. Amoebic vs pyogenic liver abscesses: A comparative study in a tertiary care hospital. J Acad Clin Microbiol [serial online] 2015 [cited 2019 Nov 20];17:89-93. Available from: http://www.jacmjournal.org/text.asp?2015/17/2/89/171889
| Introduction|| |
Liver abscess, a potentially life-threatening intra-abdominal infection, falls broadly into two categories: Amoebic liver abscess (ALA) and pyogenic liver abscess (PLA). In tropical and subtropical countries, 50-70% of cases of liver abscess are amoebic in origin.  ALA accounts for 3-9% of all cases of amoebiasis.  The incidence of PLA ranges 1.1-2.3 cases per 1,00,000 population every year. They are frequently polymicrobial, with Klebsiella pneumoniae, Escherichia coli, Streptococcus milleri and Bacteroides fragilis being the most common isolates.  About one-fourth of cases are cryptogenic. In 25% of patients, abscesses are sterile, probably due to antibiotic treatment.  The differentiation of ALA from PLA is greatly assisted by amoebic serology, as liver function tests and microscopy are non-discriminatory, facilities for amoebic culture are lacking and the positivity rate of bacterial culture is often affected by prior antibiotic therapy. The enzyme-linked immunosorbent assay (ELISA) test, which determines the anti-lectin immunoglobulin G (IgG) antibody, is the most frequently used serological test. 
At our institution in central Kerala, India, patients with liver abscess are treated successfully with percutaneous drainage, blanket therapy of antibiotics and anti-amoebic drug combinations, but the exact prevalence of invasive amoebiasis with liver abscesses remains undetected due to non-availability of serological diagnostic tests for routine screening of ALA. This study aims to find out the aetiology of liver abscess in patients admitted with specific predisposing factors, if any, and to know the proportion of ALA using the IgG ELISA test.
| Materials and methods|| |
The study was carried out at Government Medical College, Thrissur, Kerala. The study protocol was approved by the Institutional Research Committee. The study period was 6 months: From June 2012 to November 2012.
Patients with clinical features, laboratory investigations and ultrasonographic (USG) evidence of liver abscess who were admitted to the Surgery unit were included in this study.
Patients aged less than 18 years, those with organized abscess and pregnant females were excluded.
Informed consent was obtained from all the subjects involved. Detailed history, symptoms, signs, therapeutic interventions and laboratory data on admission and during the clinical course were recorded. USG-guided abscess aspirates were subjected to microscopy (wet film for trophozoites of Entamoeba histolytica, Gram stain for bacteria and acid-fast stain for Mycobacterium tuberculosis), aerobic (MacConkey and blood agar) culture and anaerobic culture (Anaerocult, Merck diagnostics, Darmstadt, Germany) for bacteria. The isolates were identified by standard methods.  Blood samples (5 mL) were collected from all patients, serum was separated and all the samples were tested for hepatitis B surface antigen (HBsAg) and antibodies to human immunodeficiency virus (HIV), hepatitis A, hepatitis C and hepatitis E by ELISA to rule out other causes of hepatic infections, before testing the samples for anti-amoebic IgG antibodies using a commercially available ELISA kit (Novalisa TM Entamoeba histolytica IgG ELISA, ENTG0140, NovaTec Immunodiagnostic GMBH, Dietzenabach, Germany). Thirty-five age, sex and locality matched control samples with no history of liver disease were also tested for anti-amoebic IgG antibodies.
ALA was defined with sterile bacterial cultures, the presence of the trophozoite of Entamoeba histolytica in wet film microscopy and/or positive ELISA test for anti-amoebic antibodies.  PLA was defined by positive bacterial cultures/infection or abdominal pathology in the distribution of the portal vein and negative amoebic serology. , Those patients with liver abscess who did not fall under either of the two groups were termed indeterminate.
Control sample size was calculated using the formula, (Z alpha + Z beta) 2 xpxqx2/d 2
where p = proportion = p1 + p2/2 (p1 = proportion of ALA in diseased and p2 = proportion of ALA in endemic areas in normal individuals, q = 100−p, d = p1−p2, and (Z alpha + Z beta) 2 = 7.14, a constant; p1 = 50, p2 = 10
Data entry and data analysis were done using Microsoft EXCEL (Washington, USA) and Epi info (CDC, Atlanta, Georgia, USA). A P value less than 0.05 was considered statistically significant when comparing the two groups with liver abscess.
| Results|| |
Of the 50 patients with liver abscess studied, 34 (68%) were diagnosed as amoebic, 11 (22%) as pyogenic and five (10%) as indeterminate. The age range was 24-85 years and 49 (98%) of the patients were males. The personal histories of the 50 patients are shown in [Table 1].
|Table 1: Main factors in the personal history of 50 patients admitted with liver abscess|
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Several demographic, behavioural, clinical and laboratory parameters were compared between the two main groups (ALA and PLA) to find out whether we can distinguish between the two, as their treatment and prognoses differ [Table 2] and [Table 3]. There was a higher association of alcohol intake with ALA compared to PLA. Findings from USG of patients with ALA and PLA are shown in [Table 4].
|Table 2: Comparison of demographic, behavioural and clinical parameters in patients with amoebic and pyogenic liver abscess|
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|Table 3: Comparison of laboratory parameters in patients with ALA and PLA|
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All the 50 cases tested for HBsAg, antibodies against HIV, and hepatitis A, C and E turned out to be negative. While 34/50 (68%) cases were positive for anti-amoebic IgG antibodies by ELISA, 4/34 (8.8%) of ALA showed motile trophozoites of Entamoeba histolytica on wet film examination. Among the 35 age-, sex- and locality-matched control samples tested, with no history of liver disease, four (11.4%) were positive for anti-amoebic antibody by ELISA.
Out of 50 cases, 11 (22%) cases were defined as PLA. One patient had a history of trauma with injury to the abdomen and had had surgical intervention 2 years back. Another gave a history of chronic pancreatitis. Samples from six cases of PLA out of 11 (54.5%) yielded bacterial isolates by aerobic culture. Polymicrobial infection (Escherichia coli and Enterococcus faecalis) was diagnosed in one patient (9%), and none of the cases showed anaerobic growth [Table 5]. Three of the five culture-negative PLA cases were complicated by ruptured liver abscesses. One of them developed pelvic abscess and another had appendicitis.
Five cases of liver abscess (10%) could not be grouped into either of the two major groups. They were negative for amoebic serology and their culture was sterile. No predisposing abdominal pathology could be found in these cases.
All the patients included in this study group had undergone percutaneous drainage. PLA was treated with broad-spectrum antibiotics, drainage and surgery with peritoneal lavage in three cases of ruptured liver abscess. There was no mortality in the pyogenic cases. The average duration of hospital stay in this group was 11 days. ALA cases were also treated with broad-spectrum antibiotics including Metronidazole and Ciprofloxacin for 10 days. One patient died in this group (2.9%) with rupture of the liver abscess into the peritoneal cavity. The average duration of hospital stay for ALA patients was 9.75 days.
| Discussion|| |
Amoebiasis is a protozoan disease that affects 10% of the world population, with a high prevalence in Mexico, South Africa, some areas in the Middle East, South Asia, Southeast Asia and parts of West Africa and South America. 
Of the 50 cases of liver abscess studied, 34 (68%) were found to be amoebic in origin by serology and 16 (22%) were pyogenic. This reinforces the findings of previous studies conducted in the tropical countries which showed higher incidence of ALA in this area compared to PLA. , ALA has a predilection for middle-aged men and is less common at extremes of age, whereas PLA is often seen in individuals >50 years. , In the present study, no significant difference could be seen between the two types of liver abscess in the age group, as 61.7% of ALA and 63.6% of PLA occurred in patients >50 years. This upward shift in the age range has been observed in earlier reports. , With the development of better diagnostic techniques, early antibiotic administration, and the improved survival of the general population, the demography has shifted towards the sixth and seventh decades of life. Male preponderance to both diseases has been reported in various studies. , The difference in gender incidence could reflect differences in environmental exposure related to occupation, although the precise underlying explanation remains unknown.
Diabetes mellitus as a risk factor for PLA has been mentioned in various studies. , However, in the present study no significant difference could be seen for diabetes as a risk factor between the two diseases under discussion. Alcohol suppresses the functioning of Kupffer cells in the liver and is a direct hepatotoxin. A high content of iron in the diet, often from country liquor in habitual drinkers, predisposes to invasive amoebiasis. , In the present study, consumption of alcohol, especially toddy, was found to be high in both groups (ALA 91.1% and PLA 54.5%). There is a higher proportion of alcohol intake in ALA when compared to PLA in our study.
Blood parameters for both types of abscesses are similar, with increased erythrocyte sedimentation rate (ESR), leucocytosis with shift to left and slight changes in liver function tests. In cases secondary to biliary tract infection, bilirubin levels are often raised. In our study, 85.2% of ALA and 90.9% of PLA cases showed leucocytosis with no significant difference. Bilirubin, liver enzymes and alkaline phosphatase levels were all raised in both groups, with no statistically significant difference. This is comparable to various other studies. ,
USG and computed tomography (CT) scanning have proved particularly useful for demonstration and drainage of abscesses. However, it is observer-dependent, and the sensitivity is 92-97%. ,, ALA usually occurs in the right lobe of the liver and is solitary in 30-70% of cases. Multiple liver abscesses occur in 4-42%, and 20-35% have abscess in the left lobe. ,, The predilection of ALA in the right lobe is because that lobe receives most of the blood draining from the right colon, the primary site of intestinal amoebiasis. Diseases of the colon, e.g., appendicitis or diverticulitis, predisposing to PLA are also very common in this region. Another factor is that the volume of blood flow is more in the right lobe and the biliary canaliculi are denser, thus leading to more congestion.  The present study did not find any correlation between the presence of either single or multiple lesions, the site of the lesion and the underlying disease. Similar results were obtained by various other studies. ,,
The sensitivity and specificity of conventional microscopy in diagnosis of Entamoeba histolytica are less than optimal, with only 11-25% of motile trophozoites visible. , In the present study, only 8.8% of ALA cases revealed motile trophozoites on wet film examination of the liver aspirate. There are several factors that adversely affect the results of microscopy. Lack of well-trained personnel; delayed deliveries to the lab; difficulty in differentiation between non-motile trophozoites, neutrophils, macrophages and tissue cells; inadequate collection conditions; and interfering substances such as antibiotics are the factors that make microscopic diagnosis quite inadequate.
The absence of serum antibodies to Entamoeba histolytica after 1 week of symptomatic liver disease is strong evidence against a diagnosis of invasive amoebiasis. They are detected in 85-95% of all patients with invasive amoebiasis. , The indirect haemaggutination (IHA) test has been replaced by commercially available ELISA kits for serodiagnosis of amoebiasis. ELISA is relatively simple, easy to perform, rapid, inexpensive, and more sensitive. Of the recommended serological tests, those that detect the presence of anti-lectin IgG antibodies are the most frequently used for diagnosis.  In the present study, after eliminating HIV and hepatitis A, B, C and E, 34 of the 50 cases (68%) with liver abscess that were sterile by bacterial culture were positive for anti-amoebic IgG antibodies. As antibodies persist for many years, ELISA or IHA cannot distinguish acute from remote infection in areas of endemicity such as ours. This may be the reason for the positivity of the four control samples (11.4%) without USG-proved liver abscess.
Of the 11 cases defined as pyogenic, which were negative for IgG anti-amoeba antibody, in six cases bacterial isolates were obtained from aerobic culture (54.5%) with both Klebsiella pneumoniae and Escherichia coli in two samples each. This is similar to the findings of another study.  In one patient (9%), polymicrobial infection was diagnosed with both Escherichia coli and Enterococcus faecalis. This is less when compared to other studies. , In five cases of PLA, culture was sterile, which may be partly due to the early antibiotic treatment. Lack of anaerobic agents may be due to lack of a good transport system, delay in processing, and early antibiotic treatment.
The treatment of ALA consists of intra-luminal and systemic administration of amoebicides as soon as possible. , Guided percutaneous drainage is performed in cases of large abscess >5 cm, those in the left lobe, and when no response to medical treatment occurs. , There is no indication for routine aspiration in ALA. A combination of USG findings with positive serology in an appropriate clinical setting is adequate to start drug treatment. In our study, all the cases of ALA underwent percutaneous drainage. They were treated with Metronidazole and Ciprofloxacin for 10 days. Open surgical drainage is rarely indicated in ALA. It is done only when the abscess has ruptured into the adjacent viscera or the peritoneum. In our study, one patient with ALA had a rupture for which exploratory laparotomy with peritoneal lavage was done. The mortality rate was 2.2% as one patient with rupture into the peritoneal cavity could not undergo surgery due to poor general condition. The rest of the patients were discharged, with an average of 9.75 days' hospital stay. After the clinical cure, the sonographic abnormality takes several months to disappear. Therefore, clinical criteria rather than USG should be used to monitor the results of the treatment. 
The treatment of choice for PLA, i.e., percutaneous drainage and antibiotics, cures 72-90% of patients.  In the present study, all patients with PLA had percutaneous drainage and were treated with broad-spectrum antibiotics. Surgery with peritoneal lavage was done in three cases of rupture with no mortality.
| Conclusion|| |
Our area is endemic for amoebiasis, thus ALA is predominant here. Alcohol intake was found to have a higher association with ALA when compared to PLA. No single clinical or biochemical parameter could be obtained to differentiate between the two diseases. No significant difference could be seen between ALA and PLA in the distribution of abscesses. Amoebic serology was found to be useful in diagnosing ALA along with clinical and radiological findings, even though the presence of remote infection could not be ruled out. As ALA is a potentially life-threatening infection, rapid diagnosis is mandatory so that prompt treatment can follow, resulting in rapid recovery. As the sensitivity of microscopy is poor, with little or no available culture, serodiagnosis in patients with suspected amoebic abscess is often an important tool in clinical decision-making. This is a pilot study with a relatively small number of cases, but it is the first of its kind in our setting and it emphasises the need to conduct further studies.
We gratefully acknowledge the invaluable help in statistical analysis by Dr. Sajna M.V, Assistant professor, Community Medicine, Govt. Medical College, Thrissur.
Financial support and sponsorship
The research was financially supported by Institutional Research Committee of Govt. Medical College, Thrissur with State Board of Medical Research (SBMR) grant.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Sifri CP, Madoff LC. Infections of the liver and biliary system. In: Mandell GL, Bennett JE, Dolin R, editors. Mandell, Douglas, and Bennett′s Principles and Practice of Infectious Diseases.7 th
ed. Edinburg: Churchill Livingstone; 2010. p. 1035-44.
Sharma N, Sharma A, Varma S, Lal A, Singh V. Amoebic liver abscess in the medical emergency of a North Indian hospital. BMC Res Notes 2010; 3:21.
Cosme A, Ojeda E, Zamarreño I, Bujanda L, Garmendia G, Echeverría MJ, et al
. Pyogenic versus amoebic liver abscess. A comparative clinical study in a series of 58 patients. Rev Esp Enferm Dig 2010;102:90-9.
Cerwenka H. Pyogenic liver abscess: Differences in etiology and treatment in Southeast Asia and Central Europe. World J Gastroenterol 2010;16:2458-62.
Tanyuksel M, Petri WA Jr. Laboratory diagnosis of amebiasis. Clin Microbiol Rev 2003;16:713-29.
Collee JG, Fraser AG, Marimon BP, Simmons A. Mackie and McCartney Practical Medical Microbiology. 14 th
ed. Delhi: Churchill Livingstone; 2006.
Lodhi S, Sarwari AR, Muzammil M, SalamA, Smego RA. Features distinguishing amoebic from pyogenic liver abscess: A review of 577 adult cases. Trop Med Int Health 2004;9:718-23.
Sharma MP, Ahuja V. Amoebic liver abscess. J Indian Acad Clin Med 2003; 4:107-11.
Mohsen AH, Green ST, Read RC, McKendrick MW. Liver abscess in adults: Ten years′ experience in a UK centre. QJM 2002;95:797-802.
Seeto RK, Rockey DC. Pyogenic liver abscess. Changes in etiology, management and outcome. Medicine [Baltimore] 1996;75:99-113.
Mukhopadhyay M, Saha AK, Sarkar A, Mukherjee S. Amoebic liver abscess: Presentation and complications. Indian J Surg 2010;72:37-41.
Jha AK, Das A, Chowdhury F, Biswas MR, Prasad SK, Chattopadhyay S. Clinicopathological study and management of liver abscess in a tertiary care center. J Nat Sci Biol Med 2015;6:71-5.
Ghosh S, Sharma S, Gadpayle AK, Gupta HK, Mahajan RK, Sahoo R, et al
. Clinical, laboratory and management profile in patients of liver abscess from northern India. J Trop Med 2014 4;2014:142382.
Abbas MT, Khan FY, Muhsin SA, Al-Dehwe B, Abukamar A, Elzouki AN. Epidemiology, clinical features and outcome of liver abscess: A single reference center experience in Qatar. Oman Med J 2014;29:260-3.
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5]